Fig. 6

Transient local reduction of Fgf signaling affects size and symmetry of developing ears. A: Localized heatshock methods. Yellow circles eggs, anterior oriented left. Untreated: wild?type development 6?24 hpf (embryos, light yellow; ears, black?outlined ellipses). Method 1 (transplant+heatshock): 6 hpf transplant donor hsp70:dnfgfr1a?EGFP cells (green) to actb2:membrane?mCherry2 host embryo; 10 hpf heatshock activates hsp70:dnfgfr1a?EGFP (red). Method 2 (local heatshock): hsp70:dnfgfr1a?EGFP;actb2:membrane?mCherry2 embryo (green). The 10 hpf localized heatshock activates hsp70:dnfgfr1a?EGFP (red). The 24 hpf treated ears smaller than untreated. B,C: Ear pairs ?24 hpf;?>?43 hpf (confocal planes); Methods 1 (B), 2 (C). Scale bar?=?50?µm. Inserts: magnifications of treated ears, GFP?expressing cells false?colored red. D?O: LR differences and B/S ratios. Untreated wild?type means (dark gray) and SD (light gray) as in Figure 3. Ear volumes: Methods 1 (D), 2 (E). Each arrow connects left (treated) and right ear values for one fish at one timepoint (four to six timepoints for three individuals per treatment; one color per individual). Squares replace arrows shorter than an arrowhead. B/S ratios for ear volumes (F). Untreated wild?type gray dots as in Figure 3; treated individuals, red (Method 1) and orange (Method 2) dots; individuals from B?E, black?outlined dots. Lumen volume (G?I), ear shape ratio (J?L), and lumen shape ratio (M?O), for individuals in D?F. See Supplementary Movies S4and S5, and Supplementary Table S2.