Fig. 5

MTP-PlexA1 inhibits developmental and tumor-specific PlexA1 pro-angiogenic role. (A) Microphotographs illustrating the expression of PlexA1 in vascular-like structures in human glioma tissues. (Scale bar: 40 μm). (B) Morpholino-based PlexA1 knockdown in Zebrafish tg(kdrl:eGFP) line. Representative images of control, Plexin-A1 mismatch and Pexin-A1 knockdown embryos (plxna1MO) are shown 28 hours post fertilization (28hpf). (C) Demonstration of the capacity of MTP-PlexA1 (10^-7M) to block VEGF (100 ng/ml)-induced migration of HMEC cells from micro-aggregates growing in a 3D plasma clot. (D) Demonstration of MTP-PlexA1 anti angiogenic effect on HUVEC cells grown 5 h on a matrigel with 10⁻⁷M of the peptide or in control (vehicle treated) condition. (E) Live-monitoring of VEGF-induced HUVEC cell migration assay using the XCelligence system (transwell assay). (F) Microphotographs illustrating the expression of PlexA1 in blood vessels of the chick chorioallontoic membrane (upper left picture). Microphotographs (middle and lower left pictures) and camera lucida drawing (middle and lower right pictures) showing representative fields of observation after 24 h incubation with VEGF (100 ng/ml, +vehicle) or VEGF + MTP-PlexA1 (10⁻⁷ M). The number of VEGF-induced new blood vessels is shown in the graph.