FIGURE

Fig. 8

ID

ZDB-FIG-180611-61

Publication

Dang et al., 2018 - Coordination of olfactory receptor choice with guidance receptor expression and function in olfactory sensory neurons

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Fig. 8

Nrp1b and robo2, but not nrp1a, are required for axonal targeting to the DZ.

Tg(BACor111-7:IRES:GAL4; UAS:citrine) (‘or111-7’) expressing OSNs project axons to the CZ in wild-type olfactory bulbs (A, D, G) while Tg(BACor130-1:IRES:GAL4; UAS:citrine) (‘or130-1’) expressing OSNs project axons to the DZ in wild type olfactory bulbs at 72 hpf (J, M, P). In nrp1a-/- larvae, or111-7 axons misproject to the DZ (B, C). Mistargeting of or111-7 axons is not observed in nrp1b-/- (E,F) or robo2-/- larvae. (H, I). However, in nrp1b-/- larvae, or130-1 axons misproject to the CZ (N, O). Similarly, robo2-/- larvae show misprojections of or130-1 axons to the MG (Q, R). Mistargeting of or130-1 axons is not observed in nrp1a-/- larvae (K,L). Fisher’s exact test (two-tailed) * = p<0.05, ** = p< = 0.005. CZ: central zone, DZ: dorsal zone, MG: medial glomerulus, LG2: lateral glomerulus 2, LG3: lateral glomerulus 3. OE: olfactory epithelium, OB: olfactory bulb.

Expression Data

Gene:	Citrine
Fish:	p201Tg; zf1062Tg p201Tg; zf1063Tg nrp1a^{sa1485/sa1485}; p201Tg; zf1062Tg nrp1a^{sa1485/sa1485}; p201Tg; zf1063Tg nrp1b^fh278/fh278; p201Tg; zf1062Tg nrp1b^fh278/fh278; p201Tg; zf1063Tg robo2^{ti272z/ti272z}; p201Tg; zf1062Tg robo2^{ti272z/ti272z}; p201Tg; zf1063Tg
Anatomical Term:	olfactory receptor cell neuron projection
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nrp1a^{sa1485/sa1485}; p201Tg; zf1062Tg nrp1b^fh278/fh278; p201Tg; zf1063Tg robo2^{ti272z/ti272z}; p201Tg; zf1063Tg
Observed In:	central zone olfactory bulb dorsal zone olfactory bulb maG1 olfactory receptor cell axon guidance olfactory receptor cell neuron projection
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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