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ZFIN ID: ZDB-FIG-180611-35
Colombo et al., 2017 - nkx genes establish SHF cardiomyocyte progenitors at the arterial pole and pattern the venous pole through Isl1 repression. Development (Cambridge, England)   145(3) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Prim-25

Fig. 2

Nkx genes delimit the OFT progenitor pool. (A-E) Dorsal view, anterior towards the top, at 26 hpf. In situ hybridization depicts diminished expression of mef2cb, which labels aSHF-derived CM progenitors, in representative nkx2.5−/−;nkx2.7+/+ (n=6) (C), nkx2.5−/−;nkx2.7+/− (n=3) (D) and nkx2.5−/−;nkx2.7−/− (n=5) (E) embryos compared with wild-type (n=15) (A) and nkx2.5+/+;nkx2.7−/− (n=5) (B) embryos. (F,G) Ventral view of the arterial pole region at 36 hpf. Confocal projections of immunohistochemistry for ZsYellow (green) and EGFP (red) in embryos carrying Tg(nkx2.5:ZsYellow) and Tg(myl7:EGFP) illustrate decreased contribution of nkx2.5+ aSHF-derived CM progenitors (ZsYellow+ EGFP) to the arterial pole in nkx2.5−/−;nkx2.7−/− (G) compared with wild-type (F) embryos (boxed areas). (H) Quantification of ZsYellow+ EGFP cells at the arterial pole in wild-type (n=9) and nkx mutant (n=8) embryos reveals a statistically significant decrease in this population following the loss of Nkx gene function. Student's t-test was used to determine statistical significance. Mean and s.e.m. of each data set are shown (**P<0.0001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu277Tg ; f2Tg ; nkx2.7vu413/vu413 standard conditions Prim-25 cardiac muscle myoblast IFL
twu277Tg ; fb7Tg standard conditions Prim-25 cardiac muscle myoblast IHC
mef2cb WT standard conditions Prim-5 heart tube ISH
nkx2.5vu179/vu179 standard conditions Prim-5 heart tube ISH
nkx2.5vu179/vu179 ; nkx2.7vu413/+ standard conditions Prim-5 heart tube ISH
nkx2.5vu179/vu179 ; nkx2.7vu413/vu413 standard conditions Prim-5 heart tube ISH
nkx2.7vu413/vu413 standard conditions Prim-5 heart tube ISH
ZsYellow nkx2.5vu179/vu179 ; f2Tg ; nkx2.7vu413/vu413 ; twu277Tg standard conditions Prim-25 cardiac muscle myoblast IHC
twu277Tg ; fb7Tg standard conditions Prim-25 cardiac muscle myoblast IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nkx2.5vu179/vu179 standard conditions Prim-5 heart tube mef2cb expression decreased amount, abnormal
nkx2.5vu179/vu179 ; f2Tg ; nkx2.7vu413/vu413 ; twu277Tg standard conditions Prim-25 cardiac muscle myoblast decreased amount, abnormal
Prim-25 cardiac muscle myoblast ZsYellow expression decreased amount, abnormal
nkx2.5vu179/vu179 ; nkx2.7vu413/+ standard conditions Prim-5 heart tube mef2cb expression decreased amount, abnormal
nkx2.5vu179/vu179 ; nkx2.7vu413/vu413 standard conditions Prim-5 heart tube mef2cb expression decreased amount, abnormal
Prim-5 heart tube mef2cb expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development