FIGURE

Fig. S4

ID

ZDB-FIG-180608-67

Publication

Eve et al., 2017 - Knockdown of Laminin gamma-3 (Lamc3) impairs motoneuron guidance in the zebrafish embryo

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Fig. S4

sgRNA injection does not affect parachordal chain (PAC) development. (A) In 50 hpf Tg(fli1a:egfp) embryos injected with 1nl of 12?15 ng/?l of sgRNA1 failure of PAC development (red asterisk) is observed in fewer than 5 hemisegments at the yolk extension per embryo (48/51). (B) A third of embryos injected with sgRNA1 and Cas9 mRNA have perturbed development of the PAC in 5 or more hemisegments at the level of the yolk extension (16/47). A, anterior; D, dorsal; hpf, hours post fertilisation.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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