FIGURE

Fig. 5

ID

ZDB-FIG-180608-118

Publication

Eckerle et al., 2017 - Progesterone modulates microtubule dynamics and epiboly progression during zebrafish gastrulation

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Fig. 5

progesterone and pregnenolone effects on YCL microtubules. (a) Anti-?-tubulin immunostaining of wild-type embryos treated with progesterone or pregnenolone at indicated concentrations, and of ethanol or DMSO treated controls. Scale bar 100 �m. (b) Quantification of progesterone and pregnenolone effects on epiboly progression, measured when control embryos reached 80% epiboly (see also supplemental Fig. S3, a and b). Embryos treated with 75 �M or 100 �M progesterone arrested gastrulation at 30?40% epiboly, marked by stop symbol (50 ? 100 �M: some pregnenolone precipitation occurred). Error bars - SD. (c) Progesterone and pregnenolone measured by ELISA in wild-type embryos incubated in 50 �M progesterone or 20 �M pregnenolone from 2 hpf onward. Pregnenolone levels increase>10-fold, pregnenolone levels>80-fold.

Expression Data

Antibody:	Ab1-tubb
Fish:	AB/TL
Conditions:	chemical treatment by environment: pregnenolone chemical treatment by environment: progesterone
Anatomical Terms:	whole organism microtubule yolk microtubule
Stage Range:	30%-epiboly to 75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TL
Condition:	chemical treatment by environment: progesterone
Observed In:	epiboly yolk microtubule yolk microtubule bundle formation yolk microtubule cytoskeleton organization
Stage Range:	30%-epiboly to 75%-epiboly

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 434(2), Eckerle, S., Ringler, M., Lecaudey, V., Nitschke, R., Driever, W., Progesterone modulates microtubule dynamics and epiboly progression during zebrafish gastrulation, 249-266, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.