Fig. 4

ache mutant embryos develop larger tumors with both Hep3B and SKHep1 human liver cancer cell xenotransplantation. At 2 dpf, embryos collected from ache+/− in-cross were injected with either Hep3B (a,b) or SKHep1 (c,d) cells. At 3 dpf, tail test was applied for phenotypically separating wild-type (a,c) and mutant embryos (b,d). At 3 dpi, larvae were fixed and imaged for analyzing tumor development (representative of Hep3B and SKHep1 injected groups, three different larvae are shown in a, a’, a”, b, b’, b” and c, c’, c”, d, d’, d”, respectively). Merged images from brightfield and GFP channels were shown. DiO labeled tumor masses can be clearly seen in green. Tumor sizes were measured in ImageJ using the fluorescent signal alone. In total 89 Hep3B injected ache+/?, 26 Hep3B injected ache−/−, 54 SKHep1 injected +/? and 20 SKHep1 injected −/− larvae were analyzed across two independent experiments. Results from each experimental set are separately graphed (e,f). Error bars represent the standard deviation. Bar = 1 mm.