FIGURE

Fig. 3

ID

ZDB-FIG-180516-5

Publication

Zhang et al., 2017 - Rb1 promotes T cell maturation from premature apoptosis by inhibiting E2F1

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Fig. 3

Excessive apoptotic early T lymphocytes in the thymus of rb1^smu8/smu8 mutants. (A) Double staining of BrdU/rag2-dsRed show BrdU incorporation of T cells in 4 dpf siblings and rb1^smu8/smu8 mutants (left). The white ovals indicate the thymus region. The white arrows indicate proliferative T cells. Scale bars: 20 μm. The graph shows the percentages of rag2:DsRed⁺ T cells that incorporate BrdU (mean± s.e.m.; ns, not significant; n=10). (B) Double staining of TUNEL/rag2-dsRed shows TUNEL incorporation by T-cells in 4 dpf siblings and rb1^smu8/smu8 mutants. The broken line outlines the thymus region. The white arrows indicate apoptotic T cells. Scale bar: 50 μm. The graph shows the percentages of rag2:DsRed⁺ T cells that incorporate TUNEL (mean±s.e.m.; ***P<0.001; n=9). (C) Confocal images of T cells (red) exhibiting cell apoptosis (overlap of TUNEL staining) of siblings and rb1^smu8/smu8 mutant embryos injected with control or pTol-lck:rb1 plasmid at 5 dpf. Scale bars: 50 μm. The graph shows the percentages of T cells (red) exhibiting cell apoptosis (overlap of TUNEL staining) in siblings and rb1^smu8/smu8 mutant embryos injected with control or pTol-lck:rb1 plasmid (mean±s.e.m.; ***P<0.001; n=10).

Expression Data

Gene:	DsRed
Fish:	zf411Tg rb1^sm11/sm11; zf411Tg
Anatomical Term:	thymus T cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rb1^sm11/sm11; zf411Tg
Observed In:	T cell apoptotic process
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

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