FIGURE

Fig. 1

ID

ZDB-FIG-180420-1

Publication

Boselli et al., 2017 - Anisotropic shear stress patterns predict the orientation of convergent tissue movements in the embryonic heart

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Fig. 1

Schematic of photoconversion experiments and of the three-dimensional analysis of early AVC morphogenesis. (A) The atrium (a) and ventricle (v) of a 36 hpf fli:kaede heart are exposed to 405 nm UV light to photoconvert the genetically encoded Kaede protein from its green to red configuration. The same heart is imaged at 36 hpf and at 48 hpf to assay the movement of the photoconverted tissue. (B,C) Maximum intensity projection of a fli:kaede heart at 36 hpf and 48 hpf, respectively. (D) AVC segmentation and analysis: the endocardium of the AVC region is segmented with a parametric surface. The color intensity of the 3D dataset is projected on the parametric surface and the AVC is unfolded for 2D visualization and quantification of the length L of the photoconverted tissue.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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