Fig. 6

Demonstration of RL-TGR MOs specificity. (a) Brightfield and fluorescence representative images from uninjected, rltgr MO1, and rltgr MO2 injected embryos population at 3 dpf. The neuromasts were assessed (white arrowheads) in the respective injected populations by observing the embryos stained with SYTOX green nucleic acid stain. There was a decrease in the number of neuromasts in the rltgr morphants (both MO1 and MO2) as compared to uninjected embryo. (b) Quantitative assessment of the neuromasts shows that there was significant decrease in the number of neuromasts in both rltgr MO1 and MO2 injected populations (**P value <0.0001; n = 10–12). (c) Measurement of the body length shows that there was significant reduction in both rltgr MO1 and MO2 injected populations as compared to uninjected ones (*P value < 0.01; n = 26–30). (d) Brightfield images of 5dpf embryo heads stained with alcian blue cartilage stain to assess cartilage development (F1 = fish #1; F2 = fish #2). No delay in the cartilage development was observed in the morphants when compared to uninjected ones. n > 10; scale bars are shown in the images.