ZFIN ID: ZDB-FIG-180315-6
Kim et al., 2017 - Zebrafish knockout of Down syndrome gene, DYRK1A, shows social impairments relevant to autism.. Molecular autism   8:50 Full text @ Mol Autism
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 14-20 to Adult

Fig. 2

Generation of dyrk1aa KO zebrafish and microcephaly phenotype analysis. a Schematic representation of the genomic structure of dyrk1aa and a 7 bp deletion generated by gene targeting with TALEN. b Predicted structures of WT and dyrk1aa KO mutant proteins in zebrafish. The 7 bp deletion resulted in a frame-shift mutation and premature termination at the kinase domain. c, d Similar brain size in WT (c) and KO fish (d) is seen at the 2-week-old stage. Fluorescent live neurons are visible in the genetic background of HuC:eGFP transgenic zebrafish. Anterior to the left, dorsal view. Scale bars 0.2 mm. e j Sections of a 3-week-old zebrafish head region were immuno-stained with an antibody against activated caspase-3. Coronal sections at the level of the eye: bright-field image (e, h) and fluorescent image (f, g, i, and j). g, j Magnification of inset in (f, i). Arrows indicate caspase-3 positive cells in the brain. Scale bars 0.2 mm. k The number of caspase-3 positive cells is increased in the brain of KO fish. Five animals for each WT and KO fish were used for the analysis. Data are presented as mean ± SEM. * p < 0.05 by Student’s t test. l, m Pictures of adult WT and KO zebrafish. dyrk1aa KO zebrafish were normal in body length and overall morphology except for a reduction of brain size. Arrowheads indicate the position of the brain in the head region. Scale bars 5 mm. n, o Photograph of dissected brains from WT and KO zebrafish, showing microcephaly phenotype in KO zebrafish. Anterior olfactory bulbs were positioned at the left, ventral view. Scale bars 0.4 mm. p, q Confirmation of microcephaly phenotype in KO zebrafish by histological examination. Dashed line in n and o indicates the relative section position used in p and q. Brain sections were stained with H&E. KO zebrafish brain had wider ventricle space than WT zebrafish. Arrows point to TeV. Scale bars 0.4 mm

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
dyrk1aakrb1/krb1 standard conditions Days 21-29 brain apoptotic process increased process quality, abnormal
Days 21-29 brain apoptotic, abnormal
Adult brain decreased size, abnormal
dyrk1aakrb1/krb1; knu3Tg standard conditions Days 14-20 brain size, normal
Acknowledgments:
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