FIGURE

Fig. 8

ID

ZDB-FIG-180306-16

Publication

Stones et al., 2017 - Zebrafish (Danio rerio) as a Vertebrate Model Host To Study Colonization, Pathogenesis, and Transmission of Foodborne Escherichia coli O157.

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Fig. 8

The zebrafish microbiota provides a barrier to E. coli colonization. (A) Following exposure of conventionalized (conv) or gnotobiotic zebrafish larvae to foodborne EHEC (10⁸ CFU/ml) for 2 h, bacterial burden was determined by dilution plating on EHEC selective agar. Individual data points, means (n = 20 for each condition), and SD are shown. Statistical significance was determined using Student’s t test (****, P < 0.0001). (B) Examples of larval homogenates derived from conventionalized fish following EHEC infection, plated on EHEC selective CHROMagar. Mauve, blue, and white colonies correspond to EHEC, other coliforms, and Proteus sp., respectively. (C) Colonization of conventionalized zebrafish with EHEC::mCherry following 2 (Ci), 8 (Cii), 16 (Ciii), and 24 h (Civ) of infection.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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