FIGURE

Fig. 7

ID

ZDB-FIG-180118-12

Publication

Kesavan et al., 2017 - CRISPR/Cas9-Mediated Zebrafish Knock-in as a Novel Strategy to Study Midbrain-Hindbrain Boundary Development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Targeted knock-in of fluorescent reporters into the pax2a locus. Expression of Venus or tRFP driven by the pax2a locus at 24 hpf. Images were taken from live embryos anesthetized in MS-222. (A) Left panel shows dorsal view of Venus expression in the MHB and otic vesicle (OV); right panel shows merged image of fluorescent and transmitted light channels. (B) Left panel shows dorsal view of tRFP expression in the MHB and otic vesicle. (C) Left panel: lateral view of an embryo expressing tRFP in the optic stalk (OS), MHB, OV, and hindbrain neurons (Hbn). Right panel: merged image of fluorescent and transmitted light channels. (D) pax2a:venus and pax2a:tRFP fish were crossed and resulting progeny was sorted into either single (Venus⁺ or tRFP⁺) or double positive (Venus⁺ and tRFP⁺) embryos at 24 hpf. Compared to single-positive siblings, double positive embryos show no morphological abnormalities. All images are maximum intensity projections covering 50 μm tissue with a Z-interval of 2 μm. Scale bar 100 μm.

Expression Data

Genes:	RFP Venus
Fish:	pax2a^tud42Tg/+ (AB) pax2a^tud43Tg/+ (AB) pax2a^tud42Tg (AB) pax2a^tud43Tg (AB) pax2a^tud42Tg/+; pax2a^tud43Tg/+ (AB)
Anatomical Terms:	hindbrain neuron midbrain hindbrain boundary optic stalk otic vesicle
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neuroanat.