FIGURE

Fig. 1

ID

ZDB-FIG-180118-1

Publication

Zhang et al., 2017 - High Resolution Imaging of DNA Methylation Dynamics using a Zebrafish Reporter

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Fig. 1

Generation of a zebrafish reporter ubiquitously expressing mCherry-MBD. (a) The structure of human MBD1, a comparison of the amino acid sequences of human and zebrafish methyl-binding domains, and the Tol2-based vector structure including promoter, the mCherry-MBD probe, and IRES-nlsEGFP. MBD, methyl binding domain; nls, nuclear localization signal; CXXC, cysteine-rich domains; TRD, transcriptional repression domain. (b) Fluorescence images of Tg(bactin2:mCherry-MBD-IRES-nlsEGFP) F1 embryos at 6, 10, and 24 hpf. Magnified images in white boxes display the mCherry-MBD channel (left) and IRES-nlsEGFP channel (right). (c) The distribution of mCherry-MBD in the adult zebrafish heart, brain, liver, ovary, and gut. The reporter is labeled as zebraRDM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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