FIGURE

Fig. 1

ID

ZDB-FIG-171227-34

Publication

Mei et al., 2014 - Functional characterization of Prickle2 and BBS7 identify overlapping phenotypes yet distinct mechanisms

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Fig. 1

pk2 knockdown leads to body curvature and IPL neurogenesis defects in zebrafish. (A)–(D) Morphology of uninjected or pk2 MO injected embryos at 28 hpf. Injection of pk2 MO leads to body curvature phenotypes at different severity, from normal like to severe. (E) and (F). pk2 Expression in the retina sections. (E) Sense control; (F) anti-sense. pk2 is enriched in the ganglion cell layer and inner nuclear layer. (G)–(H′) Retinal neurogenesis defects in pk2 knockdown embryos. (G)–(H)_ Whole eye sections; (G′)–(H′) zoom-in view of boxed areas in (G)–(H). pk2 Knockdown leads to disorganization of inner plexiform layer synaptic connections, characterized by discontinuous layers. Scale bars are 30 μm in each image.

Expression Data

Gene:	prickle2b
Fish:	WT
Anatomical Terms:	retinal ganglion cell layer retinal inner nuclear layer
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-prickle2b knu3Tg + MO3-prickle2b
Knockdown Reagent:	MO3-prickle2b
Observed In:	neural retina development retinal inner plexiform layer retinal inner plexiform layer synapse whole organism whole organism anterior-posterior axis
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 392(2), Mei, X., Westfall, T.A., Zhang, Q., Sheffield, V.C., Bassuk, A.G., Slusarski, D.C., Functional characterization of Prickle2 and BBS7 identify overlapping phenotypes yet distinct mechanisms, 245-55, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.