FIGURE

Fig. 2

ID

ZDB-FIG-171206-78

Publication

Li et al., 2017 - Males develop faster and more severe hepatocellular carcinoma than females in kras^V12 transgenic zebrafish.

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Fig. 2

The male tumors were characterized by higher level of cell proliferation, β-catenin activation and E-cadherin loss.

Representative immunohistochemical staining of liver sections from female and male wild type and kras^V12 fish (4 months old) after 10 days of doxycycline induction are shown. (A–D) Immunohistochemical staining for PCNA. (E) Quantification of PCNA nuclear staining. (F–I) TUNEL staining for apoptotic cells. (J) Quantification of apoptotic cells. (K–N) Immunohistochemical staining for β-catenin. (O) Quantification of β-catenin nuclear staining. (P–S) Immunohistochemical staining for E-cadherin. (T) Quantification of cells losing E-cadherin membrane staining. *p < 0.05; **p < 0.01; ***p < 0.001; NS: not significant. Scale bar, 20 μm for all picture panels. Examples of membrane and nuclear staining are indicated by arrows and arrowheads, respectively.

Expression Data

Antibodies:	Ab2-cdh1 Ab5-pcna Ab13-ctnnb
Fish:	WT gz32Tg
Condition:	chemical treatment by environment: doxycycline
Anatomical Terms:	hepatocyte cell outer membrane hepatocyte nucleus liver liver hepatocellular carcinoma
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gz32Tg
Condition:	chemical treatment by environment: doxycycline
Observed In:	hepatocyte cell outer membrane liver liver cell population proliferation liver hepatocellular carcinoma
Stage:	Adult

Phenotype Detail

Acknowledgments

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