Fig. 1 S1

NICD primordia do not migrate to the tail tip and deposit fewer neuromasts.

(A and A') posterior lateral line in 2.5 dpf Tg(cldnB:lynGFP) larvae. Scale bar is 100 μm. (A' and B) NICD primordia deposit fewer neuromasts. Error bars represent standard error (p<0.001=*** Student's t test). (C) 80% of NICD primordia stall and fail to reach the tail tip. (D) Notch signaling regulates posterior lateral line primordium fate versus ganglion fate (also in [Mizoguchi et al., 2011]). The NICD posterior lateral line (pLL) primordium has significantly more cells compared to wildtype at the expense of ganglion cells. In contrast, in mib1^ta52b mutants more cells are specified towards the ganglion fate and less cells contribute to the primordium. Error bars represent one-way ANOVA with Tukey pairwise comparison test (p<0.05=*, p<0.001=***). (E) Induction of Notch after the primordium and ganglion have separated leads to a significant increase in the number of cells in neuromasts (L1–4) compared to a heat-shocked sibling. Notch overexpression was induced by a 39°C heat-shock for 45 min starting at 25 hpf (L1 was still a part of the primordium). Embryos were fixed after L4, L5 deposition (~40 hpf). Error bars indicate standard error between the hs:sibling and hs:NICD neuromast cell counts from one independent experiment (p<0.001=*** Student's t test).