FIGURE

Fig. 3

ID

ZDB-FIG-171129-11

Publication

Hou et al., 2016 - Systemic inoculation of Escherichia coli causes emergency myelopoiesis in zebrafish larval caudal hematopoietic tissue

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Fig. 3

Reaction of HSPCs to different E. coli burdens after intravenous injection.

(A), Fluorescence images showing the runx1-GFP⁺ cells in Tg(runx1:en-GFP) larvae treated with different volumes of E. coli at 2 dpi (4 dpf). Scale bars, 20??m. (B), The number of runx1-GFP⁺ cells in larvae treated with 5?10?�?10³ cfu E. coli or PBS. (70.31?�?4.18 vs 71.14?�?4.42; 148.8?�?7.14 vs 137.6?�?8.65 in E. coli vs PBS group at each time points. N???7 in each group). (C), Calculation of the runx1-GFP⁺ cells in (A) (137.6?�?8.65; 194.6?�?15.63; 148.8?�?7.14; 103.4?�?8.38 in each group. N???8 in each group). (D), WISH of cmyb (red arrows) in the larval CHT treated with different volumes of E. coli at 2 dpi (4 dpf). (E), The data obtained for cmyb⁺ cells at different time points after treatment with 5?10?�?10³ cfu E. coli or PBS (102.80?�?11.67 vs 82.40?�?6.59; 114.00?�?4.97 vs 112.00?�?6.60; 121.60?�?9.70 vs 127.00?�?6.69; 129.00?�?13.71 vs 127.60?�?8.20; 203.80?�?17.06 vs 226.40?�?20.07 in E. coli vs PBS group at each time point. N?=?8 in each group). (F), Calculation of the cmyb⁺ cells in (D) (221.60?�?18.56; 268.70?�?17.77; 203.80?�?17.06; 89.30?�?12.54 in each group. N?=?10 in each group).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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