FIGURE

Fig. 5

ID

ZDB-FIG-171127-67

Publication

Lin et al., 2016 - Stathmin-like 4 is critical for the maintenance of neural progenitor cells in dorsal midbrain of zebrafish larvae

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Fig. 5

Overexpression of CA-Stmn4 induced precocious expression of elavl3 in neuronal precursor cells during neural keel stage.

Time-lapse movies were conducted 6 minute per frame around the neural keel stage (13 hpf) after heat shock (6-somite stage). During neurulation (b), neural progenitor cells converge and migrate to the dorsal midline to establish neural rod (16 hpf) from a flattened neural plate (bud stage). Here, microtubules are revealed by doublecortin-GFP fusion protein (DCX-GFP, green, indicates thread-like microtubules structure depicting the cell shape). HuC:Kaede (green) expresses within cytoplasm. Compared to control embryo (c–g), more cells with elavl3 expression (arrows) and more apoptotic cells (arrowheads) appeared in embryo overexpressing CA-Stmn4 (mCherry, red) during the neural keel stage (h–l), characterized by a disorganized and thickening tissue (b). Corresponding time points are shown on the upper-right corner. Boundaries of neural tissues are disclosed by a pair of dash lines. All snapshots shown in dorsal side and anterior to the left. Scale bars equal 25 μm for all.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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