FIGURE

Fig. 6

ID

ZDB-FIG-171031-14

Publication

He et al., 2015 - The Effects of Hsp90α1 Mutations on Myosin Thick Filament Organization

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Hypomethylation mimicking mutations at K206 and K608 have no effect on Hsp90α1 function in myosin thick filament organization.

DNA construct expressing K206R or K608R mutant, mimicking the hypomethylated state of lysine residue, was co-injected with Hsp90α1 ATP-MO into fertilized eggs of zebrafish. The injected embryos were double stained with anti-myc (9E10) and anti-MHC (F59) antibodies at 28 hpf. A-F. A mosaic pattern of rescue was detected in all myofibers expressing the Hsp90α1 K206R (A-C) or K608R (D-F) mutant. * indicates a multinucleated fast myofiber expressing K608R mutant. The anti-MHC (F59) antibody is a slow fiber specific antibody which does not label MHC expressed in fast myofibers of zebrafish embryos. G. Plot showing the number of rescued myofibers in 9 individual embryos injected with K206R, or K608R mutant. Scale bar = 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One