FIGURE

Fig. 9

ID

ZDB-FIG-171018-50

Publication

Sun et al., 2017 - Alkbh4 and Atrn Act Maternally to Regulate Zebrafish Epiboly

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Fig. 9

Epiboly and actomyosin formation are defective in MZalkbh4;MZatrn double mutant embryos. (A) Epiboly initiation and progression delay in MZalkbh4;MZatrn double mutants. (B) The quantitative data of epiboly progression at 8 hpf are shown. (C) Co-IP assay shows that endogenous Actin-NMII interaction is decreased in alkbh4 and atrn double morphant embryos. Embryos at 75% epiboly stage were harvested for lysis. (D) Confocal images of phalloidin stained embryos at 50% epiboly, and phalloidin and anti-NMII double stained embryos at 75% epiboly. (E) Quantitative measurements of actomyosin band widths in (D) at 75% epiboly stage. (F) Quantitative measurements of the marginal EVL cell length/width ratios in (D) at both 50% and 75% epiboly stages. Ne, the number of observed embryos; Nc, the number of observed cells. *, p<0.01. Scale bars: 100 μm in (A); 50 μm in (D).

Expression Data

Antibody:	Ab3-myh9
Fish:	TU atrn^tsu22/tsu22; alkbh4^tsu20/tsu20
Anatomical Term:	external yolk syncytial layer actomyosin
Stage:	75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	atrn^tsu22/tsu22; alkbh4^tsu20/tsu20
Observed In:	epiboly involved in gastrulation with mouth forming second external yolk syncytial layer actomyosin external yolk syncytial layer actomyosin structure organization external yolk syncytial layer marginal blastomere
Stage Range:	30%-epiboly to 75%-epiboly

Phenotype Detail

Acknowledgments

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