ZFIN ID: ZDB-FIG-170922-24
Xia et al., 2017 - Mediator MED23 Links Pigmentation and DNA Repair through the Transcription Factor MITF. Cell Reports   20:1794-1804 Full text @ Cell Rep.
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Shield to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Shield to Protruding-mouth

Fig. 2

Knocking Down Med23 Inhibits Pigmentation in Zebrafish

(A) Zebrafish embryos injected with Ctrl MO, Med23 MO-1, or Med23 MO-2 were analyzed. The images show dorsal views, with the anterior to the right.

(B) Melanin content of Ctrl MO, Med23 MO-1, and Med23 MO-2 embryos. ∗∗∗p < 0.001; error bars indicate SEM.

(C and D) qRT-PCR analysis of the pigmentation-related genes Trp2 (C) and Mitf (D) in zebrafish at the indicated time points. The expression was normalized to Rbl13a. Error bars indicate SEM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dct WT standard conditions Shield to Protruding-mouth whole organism RTPCR
WT + MO1-med23 standard conditions Shield to Prim-5 whole organism RTPCR
Long-pec to Protruding-mouth whole organism RTPCR
WT + MO2-med23 standard conditions Shield to Prim-5 whole organism RTPCR
Long-pec to Protruding-mouth whole organism RTPCR
mitfa WT standard conditions Shield to Protruding-mouth whole organism RTPCR
WT + MO1-med23 standard conditions Shield to Protruding-mouth whole organism RTPCR
WT + MO2-med23 standard conditions Shield to Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-med23 standard conditions Shield - Protruding-mouth whole organism mitfa expression decreased amount, abnormal
Long-pec melanocyte decreased amount, abnormal
Long-pec melanocyte decreased size, abnormal
Long-pec whole organism decreased pigmentation, abnormal
Long-pec whole organism Melanin decreased amount, abnormal
Long-pec - Protruding-mouth whole organism dct expression decreased amount, abnormal
WT + MO2-med23 standard conditions Shield - Protruding-mouth whole organism mitfa expression decreased amount, abnormal
Long-pec melanocyte decreased amount, abnormal
Long-pec melanocyte decreased size, abnormal
Long-pec whole organism decreased pigmentation, abnormal
Long-pec whole organism Melanin decreased amount, abnormal
Long-pec - Protruding-mouth whole organism dct expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.