FIGURE

Fig. 6

ID

ZDB-FIG-170921-56

Publication

Gallagher et al., 2017 - Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation

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Fig. 6

pnrc2 and the nonsense-mediated decay effector Upf1 promote decay of cyclic mRNA. Injection of low dose pnrc2 sbMO (2 ng) has little to no effect on her1 expression (A, C), contrasting with the expected her1 misexpression observed after injection of moderate dose pnrc2 sbMO (6 ng) (B). Low dose injection of upf1 sbMO (0.25 ng) also has little effect on her1 expression (D), but when combined with a low dose of pnrc2 sbMO (2 ng), her1 misexpression is observed in about 50% of injected embryos (E). The proportion affected in each condition is plotted on a bar graph that indicates significant differences between single morphants, double morphants, and controls (F). sbMO = splice-blocking MO; **** = p<0.0001.

Expression Data

Gene:	her1
Fish:	WT WT + MO2-pnrc2 WT + MO2-pnrc2 + MO2-upf1
Knockdown Reagents:	MO2-pnrc2 MO2-upf1
Anatomical Term:	segmental plate
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-pnrc2 WT + MO2-pnrc2 + MO2-upf1
Knockdown Reagents:	MO2-pnrc2 MO2-upf1
Observed In:	segmental plate
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 429(1), Gallagher, T.L., Tietz, K.T., Morrow, Z.T., McCammon, J.M., Goldrich, M.L., Derr, N.L., Amacher, S.L., Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation, 225-239, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.