ZFIN ID: ZDB-FIG-170906-5
Kenyon et al., 2017 - Active nuclear transcriptome analysis reveals inflammasome-dependent mechanism for early neutrophil response to Mycobacterium marinum. Scientific Reports   7:6505 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-5 to Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 1

Binary transgenic zebrafish model for regulatory profiling of neutrophils in response to M. marinum. (A) Driver line expressing BirA in neutrophils (i) is crossed with effector line ubiquitously expressing Avi-tagged Rangap (ii) for biotinylation of nuclei. In double transgenic fish, only the nuclei of neutrophils will be biotinylated (iii). (B) Effector line with ubiquitously expressed Avi-Cerulean-Rangap. (C) Neutrophil-specific BirA driver line with Citrine reporter. (D) Time course analysis of neutrophil infiltration of M. marinum. Embryos were injected with M. marinum at the 32-512-cell stage, fixed and immunofluorescence carried out for mpx protein at 2 dpi (i), 3 dpi (ii), 4 dpi (iii) and 5 dpi (iv). Neutrophil localisation is indicated by white arrows. (E) Neutrophil numbers in proximity to M. marinum were quantified at 2–5 dpi. Statistical significance was determined by one way ANOVA (n = 14–18, ****p < 0.0001). (F) Infection model for subsequent experiments.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Cerulean ct700aTg ; ox121Tg standard conditions Prim-5 to Day 5 whole organism IFL
Citrine ct700aTg ; ox121Tg standard conditions Prim-5 to Day 5 neutrophil IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab4-mpx IHC WT bacterial treatment by injection: Mycobacterium marinum Long-pec neutrophil
IHC Protruding-mouth neutrophil
IHC Day 4 neutrophil
IHC Day 5 neutrophil
Phenotype Details
Fish Conditions Stage Phenotype
WT bacterial treatment by injection: Mycobacterium marinum Long-pec - Day 5 neutrophil increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.