ZFIN ID: ZDB-FIG-170706-8
Wang et al., 2015 - Point mutations in KAL1 and the mitochondrial gene MT-tRNA(cys) synergize to produce Kallmann syndrome phenotype. Scientific Reports   5:13050 Full text @ Sci. Rep.
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 5

cars2 gene knockdown with Morpholinos in Danio rerio affected the migration of GnRH3 neurons.

Whole mount in situ hybridization of the control (b (b'),d,f,h) and cars2 morphants (a (a'),c,e,g) embryos with gnrh3 (a (a'),b (b')), gnrh2 (c,d), kal1a (e,f), and kal1b (g,h) probes at 56 hpf. (a,b) front views, dorsal up; (a',b',c–h) dorsal view, anterior up. Results shown were representative figures from three independent experiments and the frequency of embryos with the indicated expression patterns were shown in the bottom left corner of each panel. Red arrows indicated the affected GnRH3 neurons by knockdown cars2. Scale bar: 0.1 mm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gnrh2 AB control Long-pec tegmentum ISH
AB + MO1-cars2 standard conditions Long-pec tegmentum ISH
gnrh3 AB control Long-pec hypothalamus ISH
Long-pec olfactory bulb ISH
AB + MO1-cars2 standard conditions Long-pec olfactory bulb ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-cars2 standard conditions Long-pec gonadotrophin-releasing hormone neuronal migration to the hypothalamus disrupted, abnormal
Long-pec olfactory bulb gnrh3 expression spatial pattern, abnormal
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