Fig. 6

Exposure to a high-fat diet preferentially increases internal AT in PB stage postembryonic zebrafish. (A) Schematic diagram illustrating the experimental design. Groups were raised normally until 27 dpf, and then fed either a standard feed (Std. feed) or high-fat 5% chicken egg yolk (CEY). (B) From 27 dpf, fish were housed in glass beakers, with 80% daily water changes. (C-D‴) Representative images of 41 dpf fish fed either the Std. feed (control, C-C‴) or a high-fat diet (5% CEY, D-D‴). Arrows in C and D indicate the position at the anal fin that was quantified in G, and the position of images magnified in C″ and D″. The outlines in C′ and D′ indicate IAT (PVAT+AVAT), which was quantified in F. (E) Scatterplot indicating the increased total AT area in high-fat-fed fish (red) relative to control-fed fish (black). Lines are fitted using ordinary least squares (OLS) regression, with 95% confidence intervals. (F) Quantification of total AT area in control (black) and high-fat-fed fish (red). (G) Quantification of interstitial fluorescence. RFU=relative fluorescence unit and was normalized to background. Groups in F and G were compared using Student's t-tests. **P<0.001; ***P<0.0001. n=15 control PB stage fish, and n=8 high-fat-fed PB stage fish.