ZFIN ID: ZDB-FIG-170602-23
Garcia et al., 2017 - In Vivo Characterization of an AHR-Dependent Long Noncoding RNA Required for Proper Sox9b Expression.. Molecular pharmacology   91(6):609-619 Full text @ Mol. Pharmacol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Long-pec

Fig. 3

Comparative analysis of AHR2 functional status and slincR expression levels and spatial pattern relative to sox9b. (A) SlincR expression in 48 hpf wild-type 5D (AHR2+/+) and AHR2-null (AHR2−/−) whole embryos developmentally exposed to 0.1% DMSO (vehicle control) or 1 ng/ml TCDD (n = 3 biologic replications). Results were statistically analyzed using the GraphPad Prism 7.02 software. The data were tested for normality using the Shapiro-Wilk normality test, analyzed with two-way analysis of variance, and correction for multiple comparisons was performed using the Dunnett’s test (95% confidence intervals). Error bars indicate S.D. of the mean. **P < 0.01 compared with DMSO control. ##P < 0.01 compared with TCDD control. (B) Dorsal view of dual immunohistochemistry and in situ hybridization samples targeting sox9b-eGFP (green) and slincR (red) in 48 hpf embryos (n = 6–10 embryos). The Tg(−2421/+29sox9b:EGFPuw2) sox9b reporter line embryos were developmentally exposed to 0.1% DMSO or 1 ng/ml TCDD. In the TCDD-exposed samples, the regions with increased slincR expression had a corresponding decrease in sox9b-eGFP expression (white rectangle); e = eye, ov = otic vesicle, p = pectoral fin, fb = forebrain, mb = midbrain, and hb = hindbrain; 100 µm scale bar. All experiments were independently repeated a minimum of two times.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP uw101Tg chemical treatment by environment: dimethyl sulfoxide Long-pec pectoral fin bud IHC
uw101Tg chemical treatment by environment: dimethyl sulfoxide, chemical treatment by environment: 2,3,7,8-tetrachlorodibenzodioxine Long-pec brain IHC
Long-pec otic vesicle IHC
si:ch1073-384e4.1 WT chemical treatment by environment: dimethyl sulfoxide Long-pec whole organism RTPCR
WT chemical treatment by environment: dimethyl sulfoxide, chemical treatment by environment: 2,3,7,8-tetrachlorodibenzodioxine Long-pec whole organism RTPCR
ahr2hu3335/hu3335 chemical treatment by environment: dimethyl sulfoxide Long-pec whole organism RTPCR
ahr2hu3335/hu3335 chemical treatment by environment: dimethyl sulfoxide, chemical treatment by environment: 2,3,7,8-tetrachlorodibenzodioxine Long-pec whole organism RTPCR
uw101Tg chemical treatment by environment: dimethyl sulfoxide Long-pec Not Detected pectoral fin bud ISH
uw101Tg chemical treatment by environment: dimethyl sulfoxide, chemical treatment by environment: 2,3,7,8-tetrachlorodibenzodioxine Long-pec brain ISH
Long-pec otic vesicle ISH
Long-pec pectoral fin bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: dimethyl sulfoxide, chemical treatment by environment: 2,3,7,8-tetrachlorodibenzodioxine Long-pec whole organism si:ch1073-384e4.1 expression increased amount, abnormal
ahr2hu3335/hu3335 chemical treatment by environment: dimethyl sulfoxide Long-pec whole organism si:ch1073-384e4.1 expression decreased amount, abnormal
ahr2hu3335/hu3335 chemical treatment by environment: dimethyl sulfoxide, chemical treatment by environment: 2,3,7,8-tetrachlorodibenzodioxine Long-pec whole organism si:ch1073-384e4.1 expression decreased amount, abnormal
uw101Tg chemical treatment by environment: dimethyl sulfoxide, chemical treatment by environment: 2,3,7,8-tetrachlorodibenzodioxine Long-pec brain EGFP expression increased amount, abnormal
Long-pec brain si:ch1073-384e4.1 expression decreased amount, abnormal
Long-pec otic vesicle EGFP expression decreased amount, abnormal
Long-pec otic vesicle si:ch1073-384e4.1 expression increased amount, abnormal
Long-pec pectoral fin bud si:ch1073-384e4.1 expression increased amount, abnormal
Long-pec pectoral fin bud si:ch1073-384e4.1 expression mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular pharmacology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Mol. Pharmacol.