FIGURE

Fig. 1

ID

ZDB-FIG-170530-4

Publication

Chow et al., 2017 - In�Vivo Measurement of Glycine Receptor Turnover and Synaptic Size Reveals Differences between Functional Classes of Motoneurons in Zebrafish

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Fig. 1

The Expression of dendra2-GlyR?1 in Motoneurons, Physiology of dendra2-GlyR?1 Channels in Muscle Fibers, and Synapse Level Targeting for dendra2 Photoconversion

(A) A single motoneuron expressing dendra2-GlyR?1 (green) along with membrane targeted tdTomato (magenta), exhibiting distributed glycinergic puncta along the labeled soma and dendrites. See also Figure S1 for costaining with gephyrin.

(B) Expression of dendra2-GlyR?1 in muscle to test channel formation. Left: transgenic muscle fiber targeted for patch recording. Right: voltage-clamp recordings at the indicated holding potentials in a muscle fiber.

(C) Precise targeting with a 405-nm laser allowed us to convert synaptically localized dendra2-GlyR?1 from green (top) to red fluorescence (bottom, represented as magenta) with near single-synapse accuracy.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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