FIGURE

Fig. S8

ID

ZDB-FIG-170524-26

Publication

Lee et al., 2017 - CNBP acts as a key transcriptional regulator of sustained expression of interleukin-6

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Fig. S8

Generation of cnbp morphants. (A) Schematic of antisense morpholino oligonucleotides for cnbp in Zebrafish. (B) RT-PCR analysis of cnbp in control or cnbp morpholino-treated zebrafish embryos at 24 hpf to assess depletion efficiency. (C) Images highlighting the morphological defects of cnbp morphants during development. Scale bars, 500 μm. (D) Infiltration of neutrophils in control and cnbp morphants after LPS treatment. In cnbp-depleted zebrafish, neutrophils fail to migrate to the inflammatory foci in the yolk of LPS-injected larvae. LPS- or PBS-injected zebrafish larvae were collected at 5 h post-injection. For Sudan Black (SB) staining, the fixed larvae were permeabilized with ethanol, after which the yolk regions were stained with 0.03% SB. Migration of SB-stained neutrophils was observed under a dissecting microscope. Images were captured and analyzed using a mono-camera and NIS-Elements software, respectively. Scale bars, 500 µm, *P<0.01 (Student’s t-test). Data are representative of three independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.