ZFIN ID: ZDB-FIG-170510-23
Chatzopoulou et al., 2017 - Functional analysis reveals no transcriptional role for the glucocorticoid receptor β-isoform in zebrafish. Molecular and Cellular Endocrinology   447:61-70 Full text @ Mol. Cell. Endocrinol.
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth

Fig. 3

Images of a 3dpf zebrafish embryo generated by crossing the Tg(hsp70:Gal4) and Tg(UAS:GFP-zGRβ) transgenic lines. Embryos were heat shocked at 37 °C for 2.5 h at 1 and 2dpf. A. Transmitted light microscopy image showing representative embryo, indicating that transgenesis and heat shock treatment do not alter the morphology of zebrafish embryos. B. Fluorescence microscopy image showing expression of the GFP-zGRβ fusion protein. The green fluorescent signal is clearly present in cells in the muscles and the eyes, and in cells lining the yolk sac. C. Larger magnification fluorescence microscopy image showing expression of GFP-zGRβ in muscle tissue. The image shows that GFP-zGRβ is localized in the nuclear cell compartment.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
d1EGFP kca4Tg ; ibl40Tg heat shock Protruding-mouth eye IFL
Protruding-mouth muscle nucleus IFL
Protruding-mouth yolk anatomical region IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Molecular and Cellular Endocrinology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Molecular and Cellular Endocrinology, 447, Chatzopoulou, A., Schoonheim, P.J., Torraca, V., Meijer, A.H., Spaink, H.P., Schaaf, M.J., Functional analysis reveals no transcriptional role for the glucocorticoid receptor β-isoform in zebrafish, 61-70, Copyright (2017) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.