Fig. 4

Calcium inhibition rescues NMII overactivation at the MHBC. (A) Hypothesized role of Ca²⁺ and NMII interactions. IF, inhibition of Ca²⁺ by 2-APB results in longer MHBC cells; AND, mypt1 knockdown results in overactivation of MRLC, causing shorter and wider MHB cells; THEN, inhibition of Ca²⁺ in mypt1-knockdown embryos would rescue MHBC cell length. (B, C) Confocal images of 24-ss embryos coinjected with mGFP and mypt1 MO and treated with (B) DMSO or (C) 2-APB. (B′, C′) Magnified images from B and C. Arrowheads indicate MHBC. (D, E) Cell length quantification at the MHBC and 40 μm outside the MHBC. For statistical analysis, the Mann–Whitney U test was done. **p < 0.01, mean ± SEM. For each measurement, mypt1 MO plus DMSO (n = 11; 22 cells) and mypt1 MO plus 2-APB (n = 13; 26 cells). Scale bars, 25 μm.