FIGURE

Fig. 5

ID

ZDB-FIG-170501-3

Publication

Seberg et al., 2017 - TFAP2 paralogs regulate melanocyte differentiation in parallel with MITF

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

tfap2a and mitfa display a genetic interaction in zebrafish.

(A-I) Compared to wildtype embryos at 72 hpf (A), melanocytes in mitfa^z25/z25 mutant embryos (B) are dark but punctate, and melanocytes in mitfa^w2/z25 trans-heterozygous embryos (C) are pale, punctate, and reduced in number. While heterozygous mutation of tfap2a in the wildtype background (tfap2a^+/-) (D) does not result in a melanocyte phenotype, mitfa^z25/z25;tfap2a^+/- mutants (E) and mitfa^w2/z25;tfap2a^+/- mutants (F) display a loss of melanocytes in the ventral stripe (brackets). Similarly, the well-characterized phenotype of fewer, paler melanocytes in tfap2a^-/- null mutants (G) is more pronounced in both the mitfa^z25/z25 mutant background (H) and the mitfa^w2/z25 mutant background (I). (J, K) Counts of ventral stripe melanocytes (see brackets in A-I) showed no difference between wildtype and tfap2a^+/- embryos (J, n = 10+), while tfap2a^+/- embryos in both mitfa mutant backgrounds had significantly fewer melanocytes in the ventral stripe (K, n = 4–8). One-way ANOVA: *p<0.05, **p<0.01, ****p<0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^z25/z25 tfap2a^ts213/ts213 mitfa^w2/+; mitfa^z25/+ mitfa^w2/+; mitfa^z25/+; tfap2a^ts213/+ mitfa^w2/+; mitfa^z25/+; tfap2a^ts213/ts213 mitfa^z25/z25; tfap2a^ts213/+ mitfa^z25/z25; tfap2a^ts213/ts213
Observed In:	caudal fin melanocyte caudal fin ventral region melanocyte
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.