Fig. 6
Xvelo Has Unique Properties for Forming a Stable Matrix (A) mRNAs encoding for GFP-tagged hnRNPA1, CPEB3, Tia1, Dazap1, FUS, and FUS156E were in vitro synthesized and microinjected into the oocytes. After overnight incubation, the oocytes were imaged by laser scanning confocal microscopy. (B) mRNAs encoding for Xvelo-mCherry and GFP-tagged Bucky ball (Buc), FUS, and the PLD-swap versions of Xvelo, in which the PLD of Xvelo was replaced either by the PLD of Bucky ball (BucPLDXvelo) or the PLD of FUS (FUSPLDXvelo), were injected into the oocytes at equal concentrations and imaged after overnight incubation. (C) Internal rearrangement of fluorescent Bucky ball-GFP (Buc), and PLD-swap versions of Xvelo, Buc(PLD)Xvelo-GFP and FUS(PLD)Xvelo-GFP, after photobleaching over time. (D) The fluorescent recovery of photobleached constructs in Balbiani bodies in (C), as well as Xvelo-WT and two other biological replicates for each, is shown by quantification of fluorescence in a bleached region over time normalized by an unbleached neighboring region. See also >Figure S6. |
Reprinted from Cell, 166, Boke, E., Ruer, M., Wühr, M., Coughlin, M., Lemaitre, R., Gygi, S.P., Alberti, S., Drechsel, D., Hyman, A.A., Mitchison, T.J., Amyloid-like Self-Assembly of a Cellular Compartment, 637-50, Copyright (2016) with permission from Elsevier. Full text @ Cell