ZFIN ID: ZDB-FIG-170222-53
Lebedeva et al., 2017 - Characterization of genetic loss-of-function of Fus in zebrafish. RNA Biology   14(1):29-35 Full text @ RNA Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Day 6

Fig. 1

Generation and validation of the fus knockout zebrafish. (A) fus alleles generated by CRISPR-Cas9. A screenshot from the UCSC genome browser shows aligned sequences from heterozygous F1 animals. CRISPR target site in fus exon 3 is underlined in red. (B) An example of a PAGE gel for genotyping the Δ8 allele. M = Low molecular weight marker. Wild type (75bp) and fus−/− (67bp) products can be discriminated. (C) Quantification of fus mRNA relative to WT mRNA in 5dpf embryos. (D) Normal morphology of the WT and fus−/− embryo at 6 d post fertilization (dpf). Scale bar is 1mm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fus WT standard conditions Day 5 whole organism RTPCR
fusmz2749/mz2749 standard conditions Day 5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fusmz2749/mz2749 standard conditions Day 5 whole organism fus expression decreased amount, abnormal
Day 6 whole organism fertile, normal
Day 6 whole organism morphology, normal
Day 6 whole organism viable, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ RNA Biol.