FIGURE

Fig. 4

ID
ZDB-FIG-170221-1
Publication
Gnosa et al., 2016 - AEG-1 knockdown in colon cancer cell lines inhibits radiation-enhanced migration and invasion in vitro and in a novel in vivo zebrafish model
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Radiation enhanced invasion via AEG-1 in a zebrafish model. DiI labelled, stable AEG-1 knockdown and negative control cells of the SW480 cell line were injected into the perivitelline space of 48 h old zebrafish embryos and irradiated with 0-10 Gy. After incubation for 24 h, the numbers of cells posterior to the anal opening were manually counted. A. Dose dependent increase of invaded SW480 cells with a significant invasion after 10 Gy radiation (p=0.014). B. AEG-1 knockdown cells had a significant lower cell invasion compared to the unirradiated negative control cells (p=0.002). Radiation increased the invasion significantly upon 10 Gy radiation in the negative control cells (p=0.007) whereas no increase was seen in the AEG-1 knockdown cells. The total numbers of embryos for each group were at least 30. Data are presented as the average number of cells/embryo for each cell line ± SEM. Only significant p-values (p<0.05) are presented. NC: negative control; AEG-1: AEG-1 knockdown.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Oncotarget