ZFIN ID: ZDB-FIG-170206-29
Song et al., 2017 - REP1 inhibits FOXO3-mediated apoptosis to promote cancer cell survival. Cell Death & Disease   8:e2536 Full text @ Cell Death Dis.
Observed In:
Stage: Day 5

Fig. 1

Cell survival was impaired in the intestine of rep1/ mutant zebrafish embryos. (a and c) Dorsal view images of wild-type zebrafish (a) and rep1/ mutant zebrafish embryos (c). Lateral view images of wild-type (b) and rep1/ mutant zebrafish embryos (d) at 5 days post fertilization (dpf). (d) Defective eye morphology was detected in rep1/ mutant embryos. The length of the intestine of rep1/ mutant embryo was shortened and it was malformed (white arrow in d). (e and f) TUNEL assays with wild-type (e) and Rep1/ mutant embryos (f). Extensive TUNEL labeling was observed in the intestine of rep1/ mutant embryos. (g and h) Immunostaining with anti-caspase-3 in wild-type Tg(flk:gfp) (g) and rep1/ mutant Tg(flk:gfp) embryos (h). Apoptotic cell death was detected in the intestine of rep1/ mutant Tg(flk::egfp) embryos (red fluorescence indicated with white arrow in h) as well as in the stomach of these embryos (yellow arrow in h). Blood vessels were visualized with green fluorescence (g and h). All images were lateral views except for (a) and (c) at 5dpf. Scale bar, 200μm

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
chmrk10/rk10 standard conditions Day 5 eye decreased pigmentation, abnormal
Day 5 eye decreased size, abnormal
Day 5 intestine decreased length, abnormal
Day 5 intestine malformed, abnormal
chmrk10/rk10; s843Tg standard conditions Day 5 apoptotic process increased occurrence, abnormal
Day 5 intestine cell apoptotic, abnormal
Day 5 vasculature morphology, normal
ZFIN wishes to thank the journal Cell Death & Disease for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Death Dis.