FIGURE

Fig. 5

ID

ZDB-FIG-170110-4

Publication

Fehr et al., 2016 - A Zebrafish Model for Chlamydia Infection with the Obligate Intracellular Pathogen Waddlia chondrophila.

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Fig. 5

CLSM acquired 3D image of W. chondrophila inclusions inside the swim bladder epithelium (A) after IF staining of Waddlia (red), mitochondria (green) and DAPI staining of host cell and bacterial DNA (blue). The image shows the formation of several bacteria containing vacuoles inside epithelial cells, accompanied by the recruitment and close association with host cell mitochondria. Close-ups of single inclusions with CLSM (B) and TEM (C) reveal typical features of the chlamydial life cycle, such as transformation from the smaller infectious EB with condensed DNA (arrow) to the larger and metabolically active RB form with finely distributed chromatin (arrow head), dividing inside the vacuole. TEM images show also the morphology of infected epithelial cells of the swim bladder (D) and endothelial cells of blood vessels (E), usually containing a single perinuclear inclusion (asterisks), strongly associated with host cell mitochondria. Scale bars (A) 2 μm, (B,C,E) 1 μm and (D) 5 μm.

Expression Data

Gene:	DsRed2
Fish:	nz50Tg
Condition:	bacterial treatment by injection: Waddlia chondrophila WSU 86-1044
Anatomical Term:	neutrophil
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nz50Tg
Condition:	bacterial treatment by injection: Waddlia chondrophila WSU 86-1044
Observed In:	swim bladder neutrophil
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Microbiol