Fig. S2

Validation of the Tg(her5:CreERT2) transgenic line. A-D. Compared expression of endogenous her5 and cre mRNA revealed by in situ hybridization on whole-mount Tg(her5:CreERT2) transgenic embryos at 24hpf (A,B) and 2 dpf (C,D). Arrowheads to the PML. E. Compared expression of mCherry, GS and HuC/D in a crosssection of the TeO area of a her5^{actswitch,T(2dpf)} larva analysed at 5dpf (section plane indicated on scheme). Confocal view. mCherry expression is limited to the PML (compare with Fig.2D). F. Polyclones issued from the mosaic recombination of her5^{actswitch,T(1dpf)} embryos observed on cross-sections of the TeO at 5dpf (section plane indicated on scheme) and analysed for the glial marker GS (F3) and the neuronal marker HuC/D (F4). Confocal view. F2-F5 are high magnifications (single and merge fluorescence channels, color-coded) of the polyclone boxed in F1. Note the tight association between mCherry-positive neurons and RG (F5, purple and yellow arrows, respectively). Arrowheads to the PML in F1. G-I. Polyclones issued from the mosaic recombination of her5^{actswitch,T(1dpf)} embryos observed on horizontal sections of the adult TeO in three different animals. Note the common anterior limit of their distribution, but that the spatial distribution of these polyclones within each mCherrypositive territory varies from fish to fish. Scale bars: E and F1 20μm, F2-F4 10μm, G-I 100μm. Abbreviations: TeO: tectum opticum.