FIGURE

Fig. S2

ID
ZDB-FIG-161222-20
Publication
Lau et al., 2016 - Knockout of Zebrafish Ovarian Aromatase Gene (cyp19a1a) by TALEN and CRISPR/Cas9 Leads to All-male Offspring Due to Failed Ovarian Differentiation
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Fig. S2

Schematic representation of the EGFP and mCherry-tagged TALEN plasmids. A) The assembling strategy to insert EGFP and mCherry genes into the TALEN backbone plasmids. B) Structure of pCS2TAL3-EGFP-DD and pCS2TAL3- mCherry-RR. C). Embryos injected with in vitro transcribed mRNA of cyp19a1a-pCS2TAL3- EGFP-DD and cyp19a1a-pCS2TAL3-mCherry-RR. Both TALE proteins were translated and the yellow signal indicates co-translation of the proteins in the same cells.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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