Fig. S5
- ID
- ZDB-FIG-161111-9
- Publication
- Cortes et al., 2016 - Developmental Vitamin D Availability Impacts Hematopoietic Stem Cell Production
- Other Figures
- All Figure Page
- Back to All Figure Page
Expression of Cxcl8 receptors cxcr1/cxcr2 in zebrafish HSPCs (related to Figure 5). A. Expression analysis of FACS sorted populations showed enhanced cxcl8 expression following exposure to 1,25(OH)D3, with the biggest effect in Flk1+/cMyb+ HSPCs (cells sorted from ≥1000 embryos/condition x 2 replicates); error bars, mean ± SD. B. qPCR analysis of ZFcxcr1 and Zfcxcr2 expression shows enrichment of ZFcxcr1 in FACS sorted HSPCs (Flk1+/cMyb+) and the Flk-/cMyb- population (n=2 replicates, cells sorted from ≥1000 embryos/condition); N.D. (not detected). C. WISH analysis and phenotype distributions of embryos treated with 1,25(OH)D3 with and without the CXCL8 inhibitor SB25002 (1μM) showed co-treatment attenuated Vitamin D-mediated increases in runx1/cmyb expression (n≥50 embryos/condition). D. FACS quantification of Flk1+/cMyb+ HSPCs after exposure to 1,25(OH)D3 with and without SB25002 (Con vs 1,25(OH)D3, *p<0.01; 1,25(OH)D3 vs SB25002, *p<0.01; Con vs 1,25(OH)D3 + SB25002, N.S.; (5 embryos/sample x 4 replicates/condition; error bars, mean ± SD ). Scale bar 100μm. |