Fig. 5

Immunohistochemical staining of the zebrafish neuromasts using antibodies against acetylated α-tubulin (AcTub; green) and phosphorylated p-38 MAPK (phospho-p-38 MAPK; magenta). The visualization was achieved using a confocal microscope. a-c the trunk neuromast L2 with the hair cells affected with phosphorylated form of p-38 MAPK presented in a merged form with marked AcTub and phospho-p-38 MAPK double-staining. a'-c' the presence of phophorylated form of p-38 MAPK in the hair cells. Single hair cells with activated p-38 were found in the control individuals (a, a'). 1.5 mM H₂O₂ exposure resulted in an unequivocal increase in the volume (µm³) of phospho-p-38 MAPK in the hair cells (b, b'). 100 nM PACAP-38 1 h preincubation did not change the volume of phospho-p-38 MAPK in the hair cells as compared to that determined in the 1.5 mM H₂O₂-treated group (c, c'). N = 15/group. Scale bars 10 µm (Color figure online)