Fig. 2

Loss of ewsa promotes chromosomal instability (CIN).

(A) Representative images of mitotic cells from 27 hours post fertilization (hpf) zebrafish embryos visualized by immunohistochemistry using an anti-α-tubulin antibody (red) and DAPI (blue). (B) Percentages of cells with aberrant mitosis in zebrafish embryos at 27 hours post fertilization, as determined after immunohistochemistry using an anti-α-tubulin antibody. Experiments were repeated three times. (n = 13 to 60 mitosis from 3 to 6 embryos per genotype). *P < 0.05. (C) Representative images of chromosomes obtained from metaphase spread of 24 hpf ewsa^w/w (top) and MZ ewsa^m/m (bottom) embryos. (D) Numbers of chromosomes per cell, and percentage of cells from ewsa^w/w (blue bars) and MZ ewsa^m/m (red bars) zebrafish embryos containing that number of chromosomes. The vast majority of ewsa^w/w cells contained the normal chromosome number (50) (blue), whereas chromosome numbers in MZ ewsa^m/m mutant zebrafish cells varied from 34 to 56 chromosomes per cell (red). P < 0.05. Note that all samples were obtained from lines with wildtype tp53^w/w background.