Fig. S4
The 2Pand1 regulatory element is driving reporter expression in mouse limb ectoderm. (A-C) E10.5 and (D-G) E16.5 Tg(2Pand1::LacZ) transgenic mouse embryos. Whole embryos are presented at E10.5, and E16.5 (A, D). Whole limbs are included for E10.5, and E16.5 (B-C, E), sections are presented for E16.5 (F-G). Reporter expression is visible in the distal ectoderm of the fore- and hindlimb beginning at E10.5, with the exception of the AER (indicated by the yellow arrowheads) (A-C). By E16.5, reporter expression is presented in the entire distal ectoderm (D-G). Note the absence of reporter expression in the mesenchymal tissues at all developmental stages (B-C, F-G). AER, Apical Ectodermal Ridge. Scale bars: 1000µm in A, D; 200µm in B, C; 200µm in E; 200µm in F; 50µm in G. |
Reprinted from Developmental Biology, 417(1), Lalonde, R.L., Moses, D., Zhang, J., Cornell, N., Ekker, M., Akimenko, M.A., Differential actinodin1 regulation in zebrafish and mouse appendages, 91-103, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.