FIGURE

Fig. 5

ID

ZDB-FIG-160916-1

Publication

Kim et al., 2016 - Natural product derivative BIO promotes recovery after myocardial infarction via unique modulation of the cardiac microenvironment

Other Figures

Fig. 5

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Fig. 5

(A) Treatment of 72 hpf Tg(cmlc2:GFP) transgenic zebrafish larvae with 2.5 µM aristolochic acid (AA) for 3 hours induces cardiomyocyte death, which can be visualized as reduced heart size in treated larvae at 168 hpf. Treatment with 5 µM BIO, but not 20 mM LiCl increased ventricular size. *P < 0.05, t-test. (B) BIO treatment completely recovered survival rate in AA-treated animals. LiCl treatment increased survival rate, but not to the same degree as BIO treated animals. *p < 0.05, t-test. (C) Micrographs of the cardiac drug-treated larvae showed that BIO increased the number of proliferating cardiomyocytes in AA-treated animals, as detected by counting BrdU-immunostained cells in the GFP-positive cardiac region (cardiomyocytes = green; BrdU positive cells = red). *p < 0.05, t-test. Scale bar = 100 µm.

Expression Data

Gene:	GFP
Fish:	f1Tg
Conditions:	chemical treatment by environment: aristolochic acid A chemical treatment by environment: aristolochic acid A chemical treatment by environment: 6-bromoindirubin-3'-oxime
Anatomical Term:	heart
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	f1Tg
Conditions:	chemical treatment by environment: aristolochic acid A chemical treatment by environment: aristolochic acid A chemical treatment by environment: 6-bromoindirubin-3'-oxime
Observed In:	cardiac muscle cell proliferation cardiac ventricle
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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