FIGURE

Fig. S1

ID

ZDB-FIG-160909-8

Publication

Noonan et al., 2016 - Loss of vhl in the zebrafish pronephros recapitulates early stages of human clear cell renal cell carcinoma

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Fig. S1

Vhl^-/- have an increased amount of lipids in the distal pronephros compared to wt siblings. BODIPY 493/503 immunofluorescence was performed in live whole mount 5.5 dpf vhl^-/- and wt siblings. BODIPY 493/503 staining (green) intensity was then quantified within a ROI encompassing the distal pronephros (yellow box), using Image J. (A and B) BODIPY staining in vhl^-/- versus wt sibling. Confocal images, 10x magnification. (C) Quantification of mean BODIPY 493/503 staining intensity within the ROI in 5.5 dpf vhl^-/- versus wt siblings. (D) Ratio of BODIPY intensity in vhl^-/-/wt siblings in both the proximal and distal pronephros. Each experiment had at least 3 larvae per sample group and was performed in triplicate. Data represent mean ± s.e.m. ****P<0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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