ZFIN ID: ZDB-FIG-160811-34
Antinucci et al., 2016 - A crystal-clear zebrafish for in vivo imaging. Scientific Reports   6:29490 Full text @ Sci. Rep.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 4

Calcium imaging of visually evoked neural activity in the retina using crystal.

(a) Two-photon functional calcium imaging of amacrine cells and ganglion cell dendrites expressing GCaMP6f (green) in 4 dpf crystal Tg(elavl3:GCaMP6f) larvae (n = 8 larvae). Distance of the eye from LCD screen is 2 cm. Recordings are performed from 2-4 Z-planes (approximately 20 µm total volume thickness) at 4 Hz image acquisition rate. Dashed box shows the angles of moving gratings relative to zebrafish larva orientation. INL, inner nuclear layer; GCL, ganglion cell layer; IPL, inner plexiform layer. Scale bar is 20 µm. (b,c) Mean ΔF/F0 image of a representative calcium recording (b) followed by voxel-wise analysis of direction and orientation selectivity of visual responses (c). DSI, direction selectivity index; OSI, orientation selectivity index. (d) ΔF/F0 calcium traces during a representative tuning experiment from the 6 selected regions of interest (ROIs) shown in (b). Polar plots showing the tuning profiles (i.e., integral ΔF/F0 responses to different stimuli) of individual ROIs are reported on the right. Stimulus epochs are shown in grey. Dark arrows indicate the different directions of gratings motion. The blank-screen null condition is indicated by a ‘-’ sign.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
a4598Tg standard conditions Day 4 detection of light stimulus involved in visual perception process quality, normal
Day 4 light-activated voltage-gated calcium channel activity process quality, normal
Day 4 optokinetic behavior occurrence, normal
mitfaw2/w2; slc45a2b4/b4; mpv17a9/a9; a4598Tg standard conditions Day 4 detection of light stimulus involved in visual perception process quality, normal
Day 4 light-activated voltage-gated calcium channel activity process quality, normal
Day 4 optokinetic behavior occurrence, normal
Acknowledgments:
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