FIGURE

Fig. 3

ID

ZDB-FIG-160805-2

Publication

Spicer et al., 2016 - Targeted Mutagenesis of the Hypophysiotropic Gnrh3 in Zebrafish (Danio rerio) Reveals No Effects on Reproductive Performance

Other Figures

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Fig. 3

gnrh3 mRNA but not Gnrh3 protein detectable in gnrh3^-/- fish.

(A) Immunohistochemistry on adult coronal brain sections (Aa) using anti-zebrafish Gnrh3 Gap (green) demonstrates the presence of Gnrh3 signal in the form of somas (white arrows) and fibers in the gnrh3^+/+ pre-optic area of the brain (Ab). However, no Gnrh3 signal was found in the gnrh3^-/- pre-optic area (Ac) or in any other region of the brain. (B) In situ hybridization on adult sagittal brain sections (Bd) using gnrh3 DIG-labeled riboprobes. The anti-sense gnrh3 riboprobe demonstrated mRNA (red) in the ventral telencephalon and pre-optic area of both gnrh3^+/+ (Ba) and gnrh3^-/- (Bb) fish. The sense gnrh3 riboprobe demonstrated no gnrh3 mRNA signal in any brain regions in the gnrh3^+/+ fish (Bc). Scale bars = 50 µm.

Expression Data

Gene:	gnrh3
Antibody:	Ab2-gnrh3
Fish:	WT gnrh3^zf713/zf713
Anatomical Terms:	preoptic area preoptic area cell body preoptic area cell projection membrane ventral telencephalon
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gnrh3^zf713/zf713
Observed In:	brain preoptic area
Stage:	Adult

Phenotype Detail

Acknowledgments

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