Fig. 2

BIX01294 treatment reduces HC regeneration in the zebrafish lateral line. (A) We treated 5 days post-fertilization (dpf) Tg (brn3c:mGFP) zebrafish with neomycin for 1 h and then treated them for 24 h or 48 h with BIX01294. HCs in these fish are GFP⁺ (green), and nuclei are stained with DAPI (blue). Scale bars = 10 µm. (B) The average number of GFP⁺ cells per NM in larvae treated with or without 20 µM BIX01294 for 24 h or 48 h after neomycin damage for 1 h. Bars are mean ± SEM, and n = total number of embryos. **p < 0.001. (C) We treated 5 dpf wild-type zebrafish with neomycin for 1 h and then treated them for 24 h or 48 h with BIX01294, and HC-specific Myosin-VI immunostaining (red) was used to label HCs. Nuclei are stained with DAPI (blue). Scale bars = 10 µm. (D) The average number of Myosin-VI⁺ cells per NM in larvae treated with or without 20 µM BIX01294 for 24 h or 48 h after neomycin damage for 1 h. Bars are mean ± SEM, and n = total number of embryos. **p < 0.001.