FIGURE

Fig. 4

ID
ZDB-FIG-160630-49
Publication
Gauron et al., 2016 - Hydrogen peroxide (H2O2) controls axon pathfinding during zebrafish development
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

H2O2 controls Shh secretion. (A-D) DiO RGC labelling at 3 dpf in control embryos (A), embryos treated with Nox-i (B), Nox-i+SAG (C) or SAG (D) from 2 to 3 dpf. The maximum length of the projections was quantified (E). The n values are indicated at the bottom of each column of the graph. Representative images are shown. (F) HyPer imaging in the glucose oxidase (Gox)-treated HeLa-HyPer cells. Scale bar, 20 µm. (G) Quantification of the H2O2 levels in the control and glucose oxidase-treated HeLa-HyPer cells. Control: n=10; Gox: n=15. (H) Western blot analysis of Shh in the cell lysates (C) or culture medium (M) upon glucose oxidase treatment. (I) Quantification of the Shh precursor following Gox treatment. Shh-precursor (Gox)/Shh-precursor (control). (J) Quantification of the amount of N-terminal Shh peptide in the medium (cf Experimental Procedures) following Gox treatment. The quantifications were performed on 5 independent experiments; a representative western blot is shown in H. (K, L) Shh-GFP fluorescence subcellular distribution in control or Gox-treated cells. Scale bar, 10 µm. (M) Shh-GFP (green) colocalized with the BODIPY TR ceramide Golgi marker (red) in Gox-treated cells. The error bars represent the standard error of the mean (SEM) (*p<0.05; **p<0.01; ***p<0.001).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 414(2), Gauron, C., Meda, F., Dupont, E., Albadri, S., Quenech'Du, N., Ipendey, E., Volovitch, M., Del Bene, F., Joliot, A., Rampon, C., Vriz, S., Hydrogen peroxide (H2O2) controls axon pathfinding during zebrafish development, 133-41, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.