FIGURE

Fig. 3

ID

ZDB-FIG-160630-4

Publication

Wang et al., 2016 - Extracellular interactions and ligand degradation shape the nodal morphogen gradient

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

FCCS measurements reveal that Lefty has higher affinity to Sqt compared to Cyc.

(A) Constructs used for injection. S, signal peptide; Pro, pro-domain; Mat, mature-domain. Red arrows indicate the convertase cleavage sites. (B) Injection procedure. (C) Confocal image of an injected embryo at 30% epiboly showing the expression patterns of the fusion proteins. Scale bar represents 50 µm. (D, E, F) Representative auto- and cross-correlation functions (ACF; CCF) and fittings. (G, H, I) Individual Ln(K_d) frequency histogram and Gaussian fits (red curve). Inset, concentration plot and linear regression (red line). X axis, concentration of bound protein (C_gr(x10^-9 M)); Y axis, products of concentrations of free proteins (C_g x C_r(x10^-17 M)). n = number of data points (i.e., number of embryos).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife